Targeting construct and null allele. (A) An overview of the targeting construct and disrupted allele. Selected restriction sites are indicated. The initiator codon lies in the second coding exon and the coenzyme A binding pocket in the third coding exon. Recombination sites (loxP), the diptheria toxin A cassette (DTA) and neomycin resistance gene (NEO) are shown. The location of genotyping primers that flank the 5' loxP site are indicated by A and B.
Chandler et al. BMC Medical Genetics 2007 8:64 doi:10.1186/1471-2350-8-64