|
Primer sequences and PCR conditions used for analysis of GATA4 mutations |
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| GATA4 exons |
Exon size (bp) |
Primer |
Primer length |
Primer sequence (5'-3') |
Melting temperature |
PCR product length (bp) |
|
|
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| exon 1 |
61 |
GT4x1F |
20 |
cttgcacgtgactcccacag |
65°C |
250 |
| GT4x1R |
20 |
aagcaaaggcggagaagctc |
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| exon 2 |
1073 |
GT4x2-1F |
21 |
tctctttctgtcgttcctctt |
65°C |
600 |
| GT4x2-1R |
20 |
gcacgtagactggcgaggac |
||||
| GT4x2-2F |
21 |
ggaccatgtatcagagcttgg |
65°C |
641 |
||
| GT4x2-2R |
20 |
gccctcgcgctcctactcac |
||||
| exon 3 |
167 |
GT4x3F |
24 |
agtcagagtgaggaagagcaagag |
65°C |
541 |
| GT4x3R |
21 |
cagtttctgtgtgccgaagag |
||||
| exon 4 |
126 |
GT4x4F |
20 |
ccagccctgcctcccgttag |
65°C |
294 |
| GT4x4R |
23 |
gaggactgagagatgggcatcag |
||||
| exon 5 |
88 |
GT4x5F |
22 |
agtagccatcacatcacacagg |
65°C |
504 |
| GT4x5R |
19 |
aaagctcccaacacgttcc |
||||
| exon 6 |
149 |
GT4x6F |
20 |
gtttgtccctgccgctgatt |
65°C |
247 |
| GT4x6R |
20 |
gcagtcggcctccccacaaa |
||||
| exon 7 |
1708 |
GTx7-1F |
19 |
acaaggctatgcgtctccc |
60°C |
289 |
| GTx7-1R |
20 |
ctgagaaaatccaacacccg |
||||
| GT4x7-2F |
20 |
gcgtaatcttccctcttccc |
65°C |
291 |
||
| GT4x7-2R |
19 |
gggacaaggacatcttggg |
||||
| GTx7-3F |
20 |
gtcgacaatctggttagggg |
60°C |
281 |
||
| GTx7-3R |
20 |
gtacatggcaaacagatgcc |
||||
| GTx7-4F |
20 |
gaggatctgagaacaagcgg |
60°C |
270 |
||
| GTx7-4R |
20 |
cagctgcattttgatgaggc |
||||
| GTx7-5F |
22 |
aaattgtggggtgtgacataca |
60 °C |
577 |
||
| GTx7-5R |
22 |
gttgcagaatctctggcttttt |
||||
| GTx7-6F |
22 |
ctgtctgtctgctcctcctagc |
65°C |
586 |
||
| GTx7-6R |
22 |
acctcccagtgaagaccactaa |
||||
Reamon-Buettner et al. BMC Medical Genetics 2007 8:38 doi:10.1186/1471-2350-8-38 |
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