Effect of mutations in the 3'-UTR of GATA4 on mRNA folding. Using the program GeneQuest (Lasergene 6.0), the effect of sequence alterations on RNA folding was predicted using the first c.+500 nt in the 3'-UTR of GATA4. GeneQuest uses the Vienna RNA folding procedure, taken from Zuker's optimal RNA folding algorithm , to fold the sense strand of selected DNA regions as RNA. Compared to the reference sequence, 7/9 mutations would result in faulty RNA folding, on which five patterns here are shown. Same pattern was observed for c.+10 T > C and c.+44T > A.
Reamon-Buettner et al. BMC Medical Genetics 2007 8:38 doi:10.1186/1471-2350-8-38