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Oligonucleotides used in this study |
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| Name |
Sequence* |
Purpose |
|
|
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| Ex-1b-f |
GCGAATTCCAGTAGCCAAGGACTAGTAG |
Forward and reverse primers to make exon 1b fragment |
| Int-1b-r |
GCGGATCCAGAATTGCTCGCGCCCTTAG |
|
| Int-1b-f |
GCGGATCCAGTGAATGTGCCGCTGCAGT |
Forward and reverse primers to make exon 4 fragment |
| Int-4-r |
GCGGTACCTCGGAGAGGGAACTGTAATC |
|
| Int-4-s |
GCGGTACCTGGCTGAGTGATCAAACCGT |
Forward and reverse primers to make exon 5 fragment |
| Ex-5-r |
CGAAGCTTGGCGGCGCGTAAGGACAGG |
|
| IVS4+5G>C-f |
GAGTCCGGGTAGcAGCCAGCACGGAG |
Forward and reverse overlap PCR primers to make IVS4+5G>C mutant |
| IVS4+5G>C-r |
CTCCGTGCTGGCTgCTACCCGGACTC |
|
| IVS5-11A>G-f |
CTCCCTTGCCCCAgCCGCCCCCAGG |
Forward and reverse overlap PCR primers to make IVS5-11A>G mutant |
| IVS5-11A>G-f |
CCTGGGGGCGGcTGGGGCAAGGGAG |
|
| IVS4-1G>T-f |
CGTTTTCAtAGAAAGAT |
Forward and reverse overlap PCR primers to make IVS4-1G>T mutant |
| IVS4-1G>T-f |
ATCTTTCTaTGAAAACG |
|
| T7 promoter |
TAATACGACTCACTATAGGG |
Forward primer to pcDNA T7 promoter to detect minigene mRNA |
| PCREx-1b-f |
TGTCGGCCGTCTCCTCATCTTCC |
Forward and reverse primers to detect endogenous and minigene mRNA |
| PCREx-5-r |
TTGCGCTCCCTCTTTCTCCATTTG |
|
| GFP-f |
GACGGCAACATCCTGGGGCACAAG |
Forward and reverse primers to GFP |
| GFP-r |
CGGCGGCGGTCACGAACTCC |
|
| GAPDH-f |
TGATGACATCAAGAAGGTGGTGAAG |
Forward and reverse primers to GAPDH |
| GAPDH-r |
TCCTTGGAGGCCATGTGGGCCAT |
|
|
*Primers are 5' to 3', mutations are in lowercase | ||
Maciolek et al. BMC Medical Genetics 2006 7:59 doi:10.1186/1471-2350-7-59 |
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