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PCR and sequencing primers for the OAZ3 gene. An "F" in the name designates a forward primer, an "R" designates a reverse primer. The position given for each primer is that of the first nucleotide, in relation to the first nucleotide of the ATG start codon. |
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| Region |
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| Primer positions |
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| PCR size |
Name |
PCR Primers 5'-3' |
Name, Position |
Sequencing Primers 5'-3' |
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| Promoter |
F-P |
CTAATCAGGTCACCACTGGATCAGAGCC |
SF-P1, -518 |
ACTGGATCAGAGCC |
| -533, 16 |
R-P |
AACAACGAGGCAGCATCTTC |
SF-P2, -283 |
GGAGTCCTGAGGTGA |
| 549 bp |
SR-P1, 16 |
AACAACGAGGCAGCATCTTC |
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| EXON 1–3 |
F-1 |
CTACCTCTACCCGATCTGGTCACCA |
SF-131, -64 |
GATCTGGTCACCATACGCC |
| -76, 1446 |
R-1 |
CCTTATGTACTATGACATGATAGAAGGG |
SF-132, 231 |
CCTGGTACTGTGTATCTTCCCACCTC |
| 1522 bp |
SF-133, 788 |
CCTGGTTGTAACCATGGCA |
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| SR-131, 557 |
CACATGCGTTCCAAGATTCCATCTACC |
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| SR-132, 1446 |
CCCCTACCTATTCCCTCCCCATTC |
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| EXON 4–5, 3'UTR |
F-4 |
CAATCCCGGCACCTC |
SF-451, 2978 |
CTGGCAGATCACTCGTGGTTAG |
| 2951, 4841 |
R-4 |
GAATGCCCTCTTCTA |
SF-452, 3216 |
GAGGGAGAGGGAGCGGCCAAACA |
| 1890 bp |
SF-453, 3831 |
GAATAACCATTCATAAGTGA |
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| SF-454, 3425 |
GCTGAGGCAGCTGGATAACT |
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| (PCR fragment includes 487 bp of the 3'UTR) |
SR-451, 3443 |
GTTATCCAGCTGCCTCAGCGCG |
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| SR-452, 3966 |
CCTCACAGAATTTAAGA |
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| SR-453, 4399 |
ATGTGTCAAGGCTTCCAGCCCCTC |
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| SR-454, 4841 |
GAATGCCCTCTTCTA |
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Christensen et al. BMC Medical Genetics 2006 7:27 doi:10.1186/1471-2350-7-27 |
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