Table 5 |
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Analysis of SNPs in LMNA. Details of primers and probes used in TaqMan® analysis and PCR conditions for direct sequencing. |
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SNPs typed by TaqMan® |
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|
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SNP ID |
Position |
Forward Primer (5'-3') |
Reverse Primer (5'-3') |
TaqMan® probe (VIC) |
TaqMan® probe (FAM) |
|
|
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|
rs955383 |
2,533 |
GGCCAGGAGTTTTAGACCAAATT |
TGAGTAGCTGAGACTATAGGCTATCATG |
ATAGCAAGgCTCCGTC |
TAGCAAGaCTCCGTCTC |
|
rs2485662 |
3,971 |
ACTACCTTCTTTCTGGCTGAAACCAG |
GGGGAAGCAGGGCTGGG |
AGACCCAAtATTGGCT |
ACCCAAcATTGGCT |
|
rs517606 |
9,827 |
CGAACTCCTAGGCTCAAGTAATCC |
GGGCTACAGACTAGAAAGAGACAGAGA |
CTGGGATgTATAGGCA |
CTGGGATtTATAGGCATGA |
|
rs593987 |
16,890 |
TTGCTGTGCTGGTGCCTTT |
GAGTTGGCACTTGCAAATGTGA |
AGCCgGACTTCCT |
AGCCaGACTTCCTTG |
|
rs528636 |
19,123 |
TCTAAATTCTGAGAGCCTCCTAGTACA |
GCAACTTAGATTCCGAGCTCCTT |
AGCAGCCaTTAGC |
AGCAGCCgTTAGC' |
|
rs508641 |
20,642 |
GGAGAGAGAGGGAAAAGCATTCA |
TGACTCAGGGCTCAGGAACGT |
ACGGGGtAGAGCT |
ACGGGGcAGAGCT |
|
rs553016 |
27,366 |
GCTTGGGACTCTGGGGAG |
CTTCCACACCAGGTCGGTA |
TCCCATCgCCACCCA |
TCCCATCaCCACCCA |
|
rs4641 |
28,037 |
CGAGGATGAGGATGGAGATGA |
TCAGCGGCGGCTACCA |
TCCATCACCACCAcGTG |
CCATCACCACCAtGTG |
|
rs7339 |
29,479 |
CAGAACTGCAGCATCATGTAATCTG |
GGGTTATTTTTCTTTGGCTTCAAG |
CCTGCACgTCATGG |
CCTGCACcTCATGG |
|
rs3204564 |
30,139 |
AGGTGGAAGAAGGGAGAAGAAAG |
GCACCCCACTTGGCTTCA |
CCTAGCTTTAgACCCTGG |
CCTAGCTTTAaACCCTGG |
|
rs536857 |
31,790 |
CACTGTGGGCTGGGGAACAC |
CCTCAGCCCTCCTCCTCAAGAG |
AGCAGGCaACGTT |
AGCAGGCgACGTT |
|
|
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SNPs typed by direct sequencing |
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|
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|
SNP ID |
Position |
Primers(5'-3') |
PCR conditions |
||
|
|
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|
rs568036 |
30,669 |
These SNPs are contained in the amplicon amplified by the primers 5'-ACTGCATCCTCCTGCTCATT-3' and 5'-GGCTCCTACTTGGCCTAACA-3' |
95°C for 2 min, followed by 35 cycles of 94°C for 30 s,60°C for 30 s, and 72°C for 90 s, followed by a 20 min 72°C final extension. |
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rs568035 |
30,670 |
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rs6669212 |
30,816 |
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|
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Low et al. BMC Medical Genetics 2005 6:38 doi:10.1186/1471-2350-6-38 |
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