Additional file 1.
Table S1. Primers used for analyzing the 14 samples. Listed are the 56 different sequences that were used for amplifying two PCR products covering the SRY gene. Each primer consists of a 454-specific sequence, a 10 nt barcode unique for each sample (in bold), and a sequence for amplifying the SRY gene (italicised). The column “total reads” shows how many reads contained the first 9 nt of the corresponding barcode (plus the first 3 nt of the SRY primer to differentiate the two different PCR products), irrespective of the 10th nt of the barcode sequence. The column “total correct reads” shows how many reads contained the expected 10th nt of the corresponding barcode.
Format: XLS Size: 58KB Download file
This file can be viewed with: Microsoft Excel Viewer
Hersmus et al. BMC Medical Genetics 2012 13:108 doi:10.1186/1471-2350-13-108