Table 1

PCR primers for deletion confirmation

Gene

Primer sequence

Annealing Temperature (°C)

PCR product length (bp)


SRY

5'-GAGACTCAGACAGCGAAGTA-3'

60

3155

5'-ACGTCCAGGATAGAGTGAAG-3'

TSPY

5'-TTACCTCCGTACCATCTACC-3'

55

3396

5'-GAAGTCAGCCTCCAACTAAG-3'

RBMY

5'-CCGTTATCCTCTTCAGTCAC-3'

60

3174

5'-CATCTAGAGGCCATGCATAC-3'


Yan et al. BMC Medical Genetics 2011 12:68   doi:10.1186/1471-2350-12-68

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