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Rapid screening for chromosomal aneuploidies using array-MLPA

Jing-Bin Yan12, Miao Xu1, Can Xiong1, Da-Wen Zhou1, Zhao-Rui Ren1, Ying Huang12, Monique Mommersteeg3, Rinie van Beuningen3, Ying-Tai Wang4, Shi-Xiu Liao4, Fanyi Zeng125, Ying Wu13* and Yi-Tao Zeng12*

Author Affiliations

1 Institute of Medical Genetics, Children's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai, P.R. China

2 Key Lab of Embryo Molecular Biology, Ministry of Health, and Shanghai Lab of Embryo and Reproduction Engineering, Shanghai, P.R. China

3 PamGene International BV, 's-Hertogenbosch, The Netherlands

4 Medical Genetic Institute of Henan Province, the People's Hospital of Henan Province, Zhengzhou, P.R. China

5 Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China

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BMC Medical Genetics 2011, 12:68  doi:10.1186/1471-2350-12-68

Published: 17 May 2011

Additional files

Additional file 1:

Table S1: Probe sequence for chromosomal aneuploidy

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Additional file 2:

Figure S1: Schematic depiction of the array technology. (A) Four-well array system was used in the study. Each array contained 412 spots, and the oligonucleotide was immobilized on an aluminum-oxide substrate. The substrate had a thickness of 60 μm with long capillary pores. The diameter of an individual pore was between 100 and 200 nm. A single spot occupied about 100,000 pores of the substrate. (B) Raw image obtained on an array. These images were recorded at 500 ms, 1000 ms, 1500 ms and 3000 ms exposure time using a Cy5 filter set. This array design contained 120 probes spotted in duplicate (240 spots), including 116 universal tag-probe oligonucleotides for different chromosomes, two exogenous target ArrayControl RNA Spikes (AM1780, Ambion, Austin, TX) oligonucleotides used as a negative control for array hybridization, and two Cy5-reference oligonucleotides.

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Additional file 3:

Table S2: Gene copy numbers on array-MLPA in normal controls

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