ROC curves of detection of methylated-p16 by two MethyLight assays. The sensitivity and specificity of the MethyLight assay at various points of relative copy number in 102 tested samples were calculated according to the result of the 150-bp MSP. The cut-off points of RCN were marked by the deep-red circlets. A: For the 70-bp classic MethyLight, the area under the curve is 0.776 (95% CI: 0.677-0.874), P = 0.000. When the cut-off point of RCN was 0.0725, the sensitivity and specificity were 0.705 and 0.845, respectively. B: For the 115-bp novel MethyLight assay, the area under the curve is 0.787 (95% CI: 0.689-0.884), P = 0.000. When the cut-off point of RCN was 0.0002, the sensitivity and specificity were 0.591 and 0.983, respectively.
Zhou et al. BMC Medical Genetics 2011 12:67 doi:10.1186/1471-2350-12-67