Figure 2.

NR4A3 gene expression in human islets. RNA was isolated from four independent (islet1-4) freshly isolated human islet preparations, and NR4A3 gene expression was determined with quantitative real-time PCR in comparison with the house-keeping gene RPS13 (40S ribosomal protein S13) and insulin-receptor-substrate 2 (IRS2). RNA preparations of two adipose tissue samples were included as a positive control for NR4A3 expression analysis.