EMSA showing the physical binding of nuclear extract to GATA binding site. The arrow indicates DNA-protein complex formed when transcription factor binds to the target site. The retarded mobility of the oligonucleotide was observed in the presence of nuclear extract, lane 2. The formation of DNA-protein complex was competitively inhibited by 25- and 50-fold amount of unlabeled -206A probe, lanes 3 and 4, but not by the same amount of unlabeled -206G probe.
Liu et al. BMC Medical Genetics 2009 10:59 doi:10.1186/1471-2350-10-59