Research article
Differentially expressed alternatively spliced genes in Malignant Pleural Mesothelioma identified using massively parallel transcriptome sequencing
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* Corresponding author: Raphael Bueno rbueno@partners.org
1 The Thoracic Surgery Oncology Laboratory and the Division of Thoracic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
2 Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA
3 Enterprise Research IS and Informatics, Brigham and Women's Hospital and Partners Healthcare Inc, Boston, MA, USA
BMC Medical Genetics 2009, 10:149 doi:10.1186/1471-2350-10-149
Published: 31 December 2009Additional files
Additional file 1:
PCR Primers used to quantify expression levels of candidate exon junctions
Format: DOC Size: 66KB Download file
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Additional file 2:
Methods to calculate exon junction expression index (EJEI) using RT-PCR
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Additional file 3:
Distribution of the 70,953 exon junctions mapped by at least one transcriptome read in at least one patient sample among 5 discovery samples
Format: XLS Size: 14KB Download file
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Additional file 4:
76 highly expressed exon junction in MPM with EJEI difference > 0.1 and their mapped read numbers, EJEI, normalized EJEI and EJEI difference between MPM samples and normal lung
Format: XLS Size: 44KB Download file
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Additional file 5:
32 highly expressed exon junction in normal lung with EJEI difference > 0.1 and their mapped read numbers, EJEI, normalized EJEI and EJEI difference between normal lung and MPM samples
Format: XLS Size: 25KB Download file
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Additional file 6:
Mapping results for the 10 candidate differentially expressed exon junctions, their exon junction expression index (EJEI), EJEI difference between mesothelioma and normal samples and the RT-PCR results
Format: XLS Size: 60KB Download file
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