Figure 1.

Overview of computer-assisted image analysis scheme. The process of transforming a slide into a score begins with the pathologist capturing a digital image. A color decomposition algorithm is performed in order to isolate the DAB stain (brown areas) from the hematoxylin stain (blue areas). Next, a membrane isolation algorithm, utilizing a set of bar filters, isolates the relevant stained membrane pixels. Finally, based on these pixels, a HER2 score can be computed. The process is repeated in order to take into account the positive control.