|
Primers of biomarkers, and their source, PCR conditions, and expected size. |
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| Targets |
Primer sequence 5'-3' |
Annealing temperature |
Cycle number |
Expected size (bp) |
Reference |
|
|
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| TGF-β1 |
sens-AGACGGAATACAGGGCTT TCGATT CA |
55°C |
35 |
492 |
[73] |
| antisens-CTTGGGCTTGCGACCCAGTAGTA |
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| GFAP |
sens-GAATGGCCACTAAGGCAGTC |
58°C |
35 |
400 |
[74] |
| antisens-TGCACTCCCTCTCTCCTGTT |
|||||
| S100B |
sens-GACTCCAGCAGCAAAGGTGAC |
58°C |
35 |
211 |
[75] |
| antisens-CATCTTCGTCCAGCGTCTCCA |
|||||
| tTG |
sens-AGGCCAACCACCTGAACAAA |
60°C |
35 |
475 |
[76] |
| antisens-CATACAGGGGATCGGAAAGT |
|||||
| NF-L |
sens-AGCAGAATGCAGACATTAGCG CC |
57°C |
38 |
200 |
[77] |
| antisens-TGGTCTCTTCGCCTTCC AAGA GT |
|||||
| AβPP69 |
sens-GCACTAACTTGCACGACTAT GGCATGCTGCTGCCCTG |
70°C |
35 |
401 |
|
| antisens-GCTGGCTGCCGTCGTGG GAACTCGGACTACCTCCTCCAC A |
|||||
| AβPP75 1 |
sens-CTACCACTGAGTCTGTGGAG |
64°C |
45 |
222 |
[50] |
| antisens-GCTGGCTGCCGTCGTGG GAAACACGCTGCCACACACCGC C |
|||||
| AβPP77 0 |
sens-CTACCACTGAGTCTGTGGAG |
64°C |
45 |
242 |
|
| antisens-CTTGAGTAAACTTTGGGA TGACACGCTGCCACACACCGCC |
|||||
| GAPDH |
sens-ACCACAGTCCATGCCATCAC |
60°C |
27 |
452 |
[75] |
| antisens-TCCACCACCCTGTTGCTG TA |
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Liao et al. BMC Infectious Diseases 2008 8:84 doi:10.1186/1471-2334-8-84 |
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