Molecular epidemiology of pneumococci obtained from Gambian children aged 2–29 months with invasive pneumococcal disease during a trial of a 9-valent pneumococcal conjugate vaccine

doi:10.1186/1471-2334-8-81

BMC Infectious Diseases

Volume 8

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Antonio M
Dada-Adegbola H
Biney E
Awine T
O'Callaghan J
Pfluger V
Enwere G
Okoko B
Oluwalana C
Vaughan A
Zaman SM
Pluschke G
Greenwood BM
Cutts F
Adegbola RA

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Antonio M
Dada-Adegbola H
Biney E
Awine T
O'Callaghan J
Pfluger V
Enwere G
Okoko B
Oluwalana C
Vaughan A
Zaman SM
Pluschke G
Greenwood BM
Cutts F
Adegbola RA
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Research article

Molecular epidemiology of pneumococci obtained from Gambian children aged 2–29 months with invasive pneumococcal disease during a trial of a 9-valent pneumococcal conjugate vaccine

Martin Antonio¹ , Hannah Dada-Adegbola¹ , Ekow Biney¹ , Tim Awine¹ , John O'Callaghan¹ , Valentin Pfluger² , Godwin Enwere¹ , Brown Okoko¹ , Claire Oluwalana¹ , Adeola Vaughan¹ , Syed MA Zaman¹ , Gerd Pluschke² , Brian M Greenwood³ , Felicity Cutts¹ and Richard A Adegbola¹

¹Medical Research Council Laboratories, Banjul, The Gambia

²Swiss Tropical Institute, Basel, Switzerland

³London School of Hygiene and Tropical Medicine, UK

author email corresponding author email

BMC Infectious Diseases 2008, 8:81doi:10.1186/1471-2334-8-81


Published:	11 June 2008

Abstract

Background

The study describes the molecular epidemiology of Streptococcus pneumoniae causing invasive disease in Gambian children

Methods

One hundred and thirty-two S. pneumoniae isolates were recovered from children aged 2–29 months during the course of a pneumococcal conjugate vaccine trial conducted in The Gambia of which 131 were characterized by serotyping, antibiotic susceptibility, BOX-PCR and MLST.

Results

Twenty-nine different serotypes were identified; serotypes 14, 19A, 12F, 5, 23F, and 1 were common and accounted for 58.3% of all serotypes overall. MLST analysis showed 72 sequence types (STs) of which 46 are novel. eBURST analysis using the stringent 6/7 identical loci definition, grouped the isolates into 17 clonal complexes and 32 singletons. The population structure of the 8 serotype 1 isolates obtained from 4 vaccinated and 2 unvaccinated children were the same (ST 618) except that one (ST3336) of the isolates from an unvaccinated child had a novel ST which is a single locus variant of ST 618.

Conclusion

We provide the first background data on the genetic structure of S. pneumoniae causing IPD prior to PC7V use in The Gambia. This data will be important for assessing the impact of PC7V in post-vaccine surveillance from The Gambia.