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Open Access Research article

Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population

Xuan-Yi Wang1*, M Ansaruzzaman2, Raul Vaz3, Catarina Mondlane3, Marcelino ES Lucas4, Lorenz von Seidlein1, Jacqueline L Deen1, Sonia Ampuero5, Mahesh Puri1, Taesung Park110, GB Nair2, John D Clemens16, Claire-Lise Chaignat7, Minoarisoa Rajerison8, Farida Nato9 and Jean-Michel Fournier9

  • * Corresponding author: Xuan-Yi Wang xywang@ivi.int

  • † Equal contributors

Author Affiliations

1 The International Vaccine Institute, Seoul, Korea.

2 ICDDR,B: Centre for Health and Population Research, Dhaka, Bangladesh.

3 CHAEM, Beira, Mozambique.

4 Ministry of Health, Maputo, Mozambique.

5 Médecins Sans Frontières, Geneva, Switzerland.

6 National Institute of Child Health and Human Development, Bethesda, Maryland, USA.

7 World Health Organization, Geneva, Switzerland.

8 Institut Pasteur, Antananarivo, Madagascar.

9 Institute Pasteur, Paris, France.

10 Department of Statistics, Seoul National University, Seoul

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BMC Infectious Diseases 2006, 6:17  doi:10.1186/1471-2334-6-17

Published: 1 February 2006

Abstract

Background

Early detection of cholera outbreaks is crucial for the implementation of the most appropriate control strategies.

Methods

The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture.

Results

The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%–99%) and 89% (95% CI: 86%–93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%.

Conclusion

This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed.