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ARMS primers used to type the ovine PRNP |
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| Name |
Sequence of Primer 5'-3' |
Allele |
Length/Label |
Fragment Length |
|
|
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| PRNP_136A |
ATACATGCTGGGAAGTGC |
A136 |
18 bp/TET |
- |
| PRNP_136V |
CTACATGCTGGGAAGTGT |
V136 |
18 bp/FAM |
- |
| PRNP_154R |
ACTAACGGTACATGTTTTCAC |
R154 |
21 bp/- |
92 bp |
| PRNP_154H |
AGTTTGTAACGGTACATGTTTTgAT |
H154 |
25 bp/- |
96 bp |
| PRNP_171Q |
GGAAGTTGTTCTGGTTACTATcCT |
Q171 |
24 bp/- |
146 bp |
| PRNP_171R |
TCAGTTAAGTTGTTCTGGTTACTATtCC |
R171 |
28 bp/- |
150 bp |
| PRNP_171H |
TTCTGAGCATAAAGTTGTTCTGGTTACTATAA |
H171 |
32 bp/- |
154 bp |
|
Small letters indicate deliberately introduced mismatches (ARMS principle); underlining indicate pieces of neutral sequence that were added to elongate the primer to allow allele-discrimination by capillary electrophoresis. | ||||
Buitkamp and Semmer BMC Infectious Diseases 2004 4:30 doi:10.1186/1471-2334-4-30 |
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