PCR analysis of HSV-1 DNA replication in the presence or absence of DMSO. Total DNA was isolated from cultures of Vero cells infected with 1000 PFU of the dUTPase/LAT recombinant and the indicated hrs post-infection and amplified in the conditions described in Material and Methods. Aliquots of the amplified products were fractionated in 6% polyacrylamide gels in Tris-borate-EDTA and evaluated by densitometry.
Aguilar et al. BMC Infectious Diseases 2002 2:9 doi:10.1186/1471-2334-2-9