Experimental design used to detect the Neuraminidase-gene derived nucleic acid of A (H1N1) virus by in situ RT-PCR. Tissue fixed on electrostatically charged slides (1) was permeabilized and genomic DNA was eliminated using RNase-free DNase (2). After in situ reverse transcription (3), synthesized cDNA was amplified and detected on tissue (4). Reverse Transcription reaction product was recovered for amplification using in vitro PCR and subsequent sequencing (5) and quantification by Real Time quantitative PCR (RTqPCR) (6).
Ocadiz-Delgado et al. BMC Infectious Diseases 2013 13:20 doi:10.1186/1471-2334-13-20