RT-PCR/RFLP analysis of the NA gene of seasonal A(H1N1) influenza viruses digestion with BspHI enzyme of DNA amplicons obtained by RT-PCR targeting the neuraminidase gene of influenza A(H1N1) isolates and covering the mutation site conferring oseltamivir resistance. Tracks A to D: A(H1N1) viruses isolated in 2008; Tracks F to I: A(H1N1) viruses isolated in 2007; Tracks A, C, F, H: amplicons digested with BspHI, Tracks B, D, G, I: undigested PCR amplicons. Track E: 100-base pair marker (New England BioLabs).
Dia et al. BMC Infectious Diseases 2013 13:106 doi:10.1186/1471-2334-13-106