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This article is part of the supplement: Abstracts from the First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)

Open Access Oral presentation

PVL positive methicillin resistant Staphylococcus aureus breast abscess infection among post-partum women in Chennai, South India

Nagarajan Abimanyu1, K Arunkumar2, M Saravanan1, G Sivakumar2 and Padma Krishnan1*

Author Affiliations

1 Dept. of Microbiology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, Chennai, India

2 Madras Medical College and Govt. General Hospital, Chennai, India

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BMC Infectious Diseases 2012, 12(Suppl 1):O13  doi:10.1186/1471-2334-12-S1-O13


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2334/12/S1/O13


Published:4 May 2012

© 2012 Abimanyu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Breast abscess and mastitis caused by MRSA is on the rise. This could result in poor response to routinely used β-lactam antibiotics. Lack of MRSA screening could lead to wrong choice of antibiotic after drainage resulting in multiple and recurrent abscesses leading to increased morbidity and mortality.

Methods

A total of 20 isolates of Staphylococcus aureus were isolated from 25 cases of breast abscess from a tertiary hospital during January 2010-October 2010. Isolation was done by culturing the pus sample drained from the abscess with 16 gauge needle. The S. aureus isolates were screened for susceptibility to various antibiotics including cefoxitin, clindamycin, fusidic acid, mupirocin, vancomycin and linezolid. MIC to oxacillin, erythromycin and clindamycin were performed using E-strip method for resistant isolates. Molecular detection of methicillin resistance and Panton-Valentine Leukocidin (PVL) were done by multiplex PCR targeting femA, mecA, and lukS-PV.

Results

Of the 20 isolates, 10 (50%) were found to be MRSA, of which 3 (30%) isolates were found to be positive for PVL. Of the tested isolates, highest sensitivity (100%) was observed for vancomycin, linezolid, mupirocin, clindamycin and fusidic acid. Highest resistance was observed for co-trimoxazole>erythromycin>ofloxacin> netilmicin>gentamicin>amikacin. High-level methicillin resistance (MIC = 256µg/mL) was observed for 3 MRSA isolates from hospitalized patients.

Conclusion

From the above results, the PVL-MRSA infections in breast abscess among post partum women is found to be increasing. Proper drainage and routine screening of MRSA from breast abscess should be done to decrease the morbidity and mortality.