Table 1 |
||||
| Parameters during each round of the SELEX assay | ||||
| SELEX rounds | Antibody (μg/well) | ssDNA(μg/well) | Reaction time in BSA-blocked blank wells (min) | Reaction time in antibody-coated wells (min) |
| 1 | 1 | 0.5 | 15 | 60 |
| 2 | 0.5 | 0.5 | 20 | 60 |
| 3 | 0.25 | 0.5 | 20 | 60 |
| 4 | 0.25 | 0.1 | 30 | 45 |
| 5 | 0.1 | 0.1 | 30 | 45 |
| 6 | 0.25 | 0.1 | 30 | 45 |
| 7 | 0.1 | 0.1 | 45 | 30 |
| 8 | 0.1 | 0.05 | 45 | 30 |
| 9 | 0.05 | 0.05 | 45 | 30 |
| 10 | 0.05 | 0.025 | 60 | 20 |
| 11 | 0.025 | 0.025 | 60 | 20 |
| 12 | 0.025 | 0.01 | 60 | 15 |
Zhu et al. BMC Infectious Diseases 2012 12:96 doi:10.1186/1471-2334-12-96
