R-848 alters Toll-like receptor expression in vitro. Freshly isolated monocytes from uninfected (n = 3) and HIV-infected (n = 5) subjects were cultured in the presence of R-848 or media alone for 24 h. TLR7/8 mRNA then quantified using real-time PCR. R-848 stimulation significantly decreased expression of TLR7 in monocytes (ap = 0.025) but did not alter TLR8 expression (bp = 0.944). Data was analyzed using the Wilcoxon signed ranks test. Bars indicate the interquartile range (IQR).
Nian et al. BMC Infectious Diseases 2012 12:5 doi:10.1186/1471-2334-12-5