Table 4

PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant organisms, plus coagulase negative staphylococci (CNS), contaminating high-use surfaces
Bacteria PCR/ESI-TOF-MS (# isolates) n = 305 TCM (# isolates) n = 89
Bedrail Door handle Faucet IV pump Keyboard Mouse Shears Bedrail Door handle Faucet IV pump Keyboard Mouse Shears
CNS 24 31 28 23 30 22 19 11 6 14 9 16 9 7
Enterococci* 2 1 2 1 1
S. aureus 4 1 4 2 3 3 1 1 1 2
Streptococci** 7 8 12 12 17 7 5 1
Acinetobacter spp.*** 2 1 2 6 1 1
Enterobacter spp.**** 3 1 1 1 1 1
E. coli 2 1 1
K. pneumoniae 7 1 3 1 3
Pseudomonas spp.***** 4 2 1
Candida spp.****** 7 3 5 3 1 2

*TCM: “Enterococcus spp.”; PCR/ESI-TOF-MS: 2 Enterococcus faecalis, 1 Enterococcus faecium.

**TCM: 1 viridans group streptococci; PCR/ESI-TOF-MS: 1 Group D Streptococcus, 24 S. agalactiae, 8 Streptococcus pneumoniae, 1 S. pyogenes, 1 “Streptococcus spp.”, 33 viridans group streptococci.

***TCM: 2 A. baumannii; PCR/ESI-TOF-MS: 10 A. baumannii, 1 “Acinetobacter spp.”

****TCM: 2 Enterobacter cloacae; PCR/ESI-TOF-MS: 4 E. cloacae; 2 Enterobacter sakazakii.

*****PCR/ESI-TOF-MS: 1 P. aeruginosa, 2 Pseudomonas entomophila/putida, 3 P. fluorescens, 1 “Pseudomonas spp.”

******PCR/ESI-TOF-MS: 10 C. albicans, 4 C. glabrata, 1 Candida kefyr, 1 Candida kruseii, 1 Candida parapsilosis, 1 Candida rugosa, 3 Candida tropicalis.

Yun et al.

Yun et al. BMC Infectious Diseases 2012 12:252   doi:10.1186/1471-2334-12-252

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