Leptin mRNA expression was significantly decreased after 6 and 24 hours of stimulation with 1 ng/ml TNF alpha (p < 0.05) in human adipocytes. Drotrecogin alpha (activated) (DAA) dose-dependently increased leptin mRNA levels after 24 hours from DAA 50 ng/ml to DAA 5 μg/ml (p < 0.05), but at 6 hours no significant change could be detected. Significances (n.s. = not significant) of t-tests with Holm-Bonferroni adjustments for multiple comparisons are indicated. Only the relevant in advance specified group comparisons were analysed. Data are presented as mean with standard error of mean (SEM).
Behnes et al. BMC Infectious Diseases 2012 12:217 doi:10.1186/1471-2334-12-217