Th1/Th2 Cytokine Standard curves generated by BD CBA Software. Human Th1/Th2 Cytokine Standards were reconstituted in 2.0 ml of Assay Diluent; dilute the Standards primary liquid in a serial dilution rate as described: 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, and 1:256; prepare one tube containing Assay Diluent as negative control (0 pg/ml). 50 μl of the mixed Capture Beads, 50 μl of the Human Th1/Th2 PE Detection Reagent, and 50 μl of Cytokine Standard dilutions were mixed before acquiring by CellQuest software; standard curves were calculated using the BD CBA Software after acquiring, sample cytokine concentrations were calculated by standard curves.
Kang et al. BMC Infectious Diseases 2012 12:102 doi:10.1186/1471-2334-12-102