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The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes

Edgar Abadia1, Jian Zhang1, Viviana Ritacco2, Kristin Kremer3, Raymond Ruimy4, Leen Rigouts5, Harrison Magdinier Gomes6, Atiná Ribeiro Elias6, Maryse Fauville-Dufaux7, Karolien Stoffels7, Voahangy Rasolofo-Razanamparany8, Darío Garcia de Viedma109, Marta Herranz109, Sahal Al-Hajoj11, Nalin Rastogi12, Carlo Garzelli13, Enrico Tortoli14, Philip N Suffys6, Dick van Soolingen153, Guislaine Refrégier1 and Christophe Sola116*

Author Affiliations

1 Institute of Genetics and Microbiology UMR8621, CNRS Université Paris-Sud 11 Universud, Campus d'Orsay, F-91405 Orsay-Cedex, France

2 Instituto Nacional de Enfermedades Infecciosas ANLIS Carlos Malbrán, Vélez Sarsfield 563, 1281 Buenos Aires, Argentina

3 National Institute for Public Health and the Environment, Bilthoven, The Netherlands

4 EA 3964 Université Paris-Diderot & Microbiology Laboratory, Bichat-Claude Bernard Hospital AP-HP, Paris, France

5 Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, 155 National Straat, 200 Antwerp, Belgium

6 Laboratory of Molecular Biology applied to Mycobacteria, Oswaldo Cruz Institute, Rio de Janeiro, Brazil

7 National Reference Centre of Tuberculosis and Mycobacteria, Scientific Institute of Public Health, Brussels, Belgium

8 Unité des Mycobactéries, Institut Pasteur de Madagascar, Antananarivo, Madagascar

9 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital Gregorio Marañón, Madrid, Spain

10 CIBER Enfermedades Respiratorias (CIBERES), Spain

11 Department of Comparative Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia

12 Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory, Institut Pasteur de Guadeloupe, Abymes, Guadeloupe

13 Dipartimento di Patologia Sperimentale Biotecnologie Mediche Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa, Italy

14 Regional Reference Center for Mycobacteria, Careggi Hospital, viale Morgagni 85, 50134 Firenze, Italy

15 Department of Pulmonary Diseases and Department of Microbiology, Radboud University Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands

16 Unité de Génétique Mycobactérienne, Institut Pasteur, 25-28 rue du Dr. Roux, F-75724 Paris-Cedex 15, France

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BMC Infectious Diseases 2011, 11:110  doi:10.1186/1471-2334-11-110

Published: 28 April 2011

Additional files

Additional file 1:

Center's distribution of problematic spacers. This file highlights the problematic spacers shared by several centers (lines).

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