Additional file 2.

Clustering of HIV nef IDU sequences. Representative phylogenetic tree, constructed by the CLUSTAL W program, using the neighbor-joining method. The tree is based on the entire nef gene sequence of 15 HIV-1 strains from IDU in Karachi, Pakistan (enclosed in the red box) and 66 HIV-1 sequences belonging to various HIV-1 subtypes deposited in the Los Alamos database.

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Rai et al. BMC Infectious Diseases 2010 10:7   doi:10.1186/1471-2334-10-7