Comparative ELISA analysis of recombinant proteins with commercial assay with patient sera negative for M. pneumoniae infection. Comparative ELISA analysis of purified recombinant P116(N-27), P1(C-40) and P116(N-27) + P1(C-40) with commercial ELISA for IgM antibodies to M. pneumoniae with 31 negative patient sera. For each serum the "blue square" shows reactivity with the P116(N-27), whereas "purple, yellow and green squares" show reactivity with the P1(C-40), P116(N-27) + P1(C-40) and commercial ELISA kit respectively (Inset shows western blot results for the three patients negative by commercial ELISA, but positive in P116(N-27), or P1(C-40) or P116(N-27)+ P1(C-40) ELISAs as well as in western blot with P116(N-27)).
Tabassum et al. BMC Infectious Diseases 2010 10:350 doi:10.1186/1471-2334-10-350