Table 2

ZFN targeting and cleaving within the adjacent 8,954 bp to the 5′ end of the hemoglobin, beta (βA)-gene
Zinc Finger Nuclease (ZFN) Left Fn; triplet- α-Helix Right Fn; triplet- α-Helix
ZFN-unknown-SP-6-1
5′_4231 aGCCACCACCTTCT GATAGGCAGCCt 4256_′3 F1; VPSKLKR; (GGC) F1; DPSTLRR; (GCC)
5′_4231 tCGGTGGTGGAAGA CTATCCGTCGGa 4256_′3 F2; EAHHLSR; (GGT) F2; QSTTLKR; (GCA)
F3; IRHHLKR; (GGT) F3; RRDGLAG; (TAG)
ZFN-unknown-SP-6-2
5′_4234 cACCACCTTCTGAT AGGCAGCCTGCa 4259_′3 F1; MKHHLAR; (GGT) F1; RGRNLEM; (TGC)
5′_4234 gTGGTGGAAGA CTATCCGTCGGACGt 4259_′3 F2; EAHHLSR; (GGT) F2; DSSVLRR; (GCC)
F3; QDGNLTR; (GAA) F3; QGGTLRR; (GCA)

Wayengera

Wayengera BMC Blood Disorders 2012 12:5   doi:10.1186/1471-2326-12-5

Open Data