Table 1 |
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E. coli strains used in this study |
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Bacteria |
Characteristics |
Source |
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AAEC185 (-) |
Derived from E. coli K-12 strain, MM294, in which the fim operon is deleted. Also recA- [34]. |
Scott J. Hultgren, Washington Univ. |
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AAEC185/pSH2 |
The AAEC185 strain with the entire fim operon expressed from the pSH2 plasmid [35]. |
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AAEC072 |
Derived from E. coli K-12 strain, MG1655. Deletion of fimB-H [34,36]. |
Ian Blomfield, Univ. of Kent |
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MG1655 |
Wild type E. coli K-12 (Fim+, but most cells are phased off for fimbrial expression |
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KB91 |
Derived from E. coli K-12 strain, MG1655, where the fim operon was deleted to produce AAEC191A [34]. The fimbrial M1L variant of FimH derived from intestinal E. coli was then expressed rom AAEC191A [25]. |
Evgeni V. Sokurenko, Univ. of Washington |
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Nu14 |
E. coli cystitis isolate that expresses the M1H variant of FimH [37]. |
Evgeni V. Sokurenko Univ. of Washington |
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J96 |
E. coli serotype O4:K6:45, a uropathogenic pyelonephritis isolate that expresses the M1H variant of FimH and P (PapG1 and G3) fimbriae. |
Obtained from American Type Culture Collection (ATCC). |
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Yu and Lowe BMC Gastroenterology 2009 9:58 doi:10.1186/1471-230X-9-58 |
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