Research article

Phospholipids reduce gastric cancer cell adhesion to extracellular matrix in vitro

Marc Jansen¹ , Karl-Heinz Treutner¹ , Britta Schmitz¹ , Jens Otto¹ , Petra Lynen Jansen² , S Neuss³ and Volker Schumpelick¹

¹  Department of Surgery, University Clinic, Pauwelsstr. 30, 52057 Aachen, Germany

²  Interdisciplinary Centre of Clinical Research (IZKF) Biomat; University Clinic, Pauwelsstr. 30, 52057 Aachen, Germany

³  Institute of Pathology, University Clinic, Pauwelsstr. 30, 52057 Aachen, Germany

author email corresponding author email

BMC Gastroenterology 2004, 4:33doi:10.1186/1471-230X-4-33

Published:	29 December 2004

Abstract

Background

Nidation of floating tumour cells initiates peritoneal carcinosis and limits prognosis of gastro-intestinal tumours. Adhesion of tumour cells to extracellular matrix components is a pivotal step in developing peritoneal dissemination of intraabdominal malignancies. Since phospholipids efficaciously prevented peritoneal adhesion formation in numerous animal studies we investigated their capacity to reduce adhesions of gastric cancer cells to extracellular matrix components (ECM).

Methods

Human gastric cancer cells (NUGC-4, Japanese Cancer Research Resources Bank, Tokyo, Japan) were used in this study. Microtiter plates were coated with collagen IV (coll), laminin (ln) and fibronectin (fn). Non-specific protein binding of the coated wells was blocked by adding 1% (w/v) BSA (4°C, 12 h) and rinsing the wells with Hepes buffer. 50.000 tumour cells in 100 μl medium were seeded into each well. Beside the controls, phospholipids were added in concentrations of 0.05, 0.1, 0.5, 0.75 and 1.0/100 μl medium. After an incubation interval of 30 min, attached cells were fixed and stained with 0.1% (w/v) crystal violet. The dye was resuspended with 50 μl of 0.2% (v/v) Triton X-100 per well and colour yields were then measured by an ELISA reader at 590 nm. Optical density (OD) showed a linear relationship to the amount of cells and was corrected for dying of BSA/polystyrene without cells.

Results

The attachment of gastric cancer cells to collagen IV, laminin, and fibronectin could be significantly reduced up to 53% by phospholipid concentrations of 0.5 mg/100 μl and higher.

Conclusion

These results, within the scope of additional experimental studies on mice and rats which showed a significant reduction of peritoneal carcinosis, demonstrated the capacity of phospholipids in controlling abdominal nidation of tumour cells to ECM components. Lipid emulsions may be a beneficial adjunct in surgery of gastrointestinal malignancies.