Table 2

Kinetic constants (Maximal velocity – Vmax- and the affinity transporter constant – Kt-) for galactose and amino acid uptake by BBMVs in the three experimental groups:
Nutrient
 Kinetic constants
 Control group (CO, n = 10)
 Untreated cirrhotic rats (CI, n = 10)
 Cirrhotic rats treated with IGF-I (CI+IGF, n = 10)
D-galactose
 Vmax
 3470 ± 30
 2460 ± 24***
 3008 ± 45*

Kt
 0.71 ± 0.01
 1.03 ± 0.02**
 0.78 ± 0.02
L-leucine
 Vmax
 305 ± 19
 210 ± 15**
 283 ± 18

Kt
 1.6 ± 0.1
 2.0 ± 0.2**
 1.1 ± 0.1
L-proline
 Vmax
 165 ± 32
 92 ± 7
 159 ± 28

Kt
 1.9 ± 0.2
 3.3 ± 0.2***
 2.1 ± 0.1
L-glutamic acid
 Vmax
 828 ± 23
 545 ± 13***
 789 ± 18

Kt
 0.6 ± 0.0
 1.1 ± 0.0**
 0.8 ± 0.0
L-cysteine
 Vmax
 195 ± 26
 105 ± 11***
 182 ± 19

Kt
 1.9 ± 0.1
 2.3 ± 0.2**
 1.9 ± 0.1

Values are mean ± SEM, n = pool of BBMVs from 10 animals from each group. Vmax expressed as pmol of substrate/ mg protein × 3 seconds and Kt as mmol/l. *p < 0.05, **p < 0.01, ***p < 0.001 CO vs other groups.

Castilla-Cortázar et al. BMC Gastroenterology 2004 4:12   doi:10.1186/1471-230X-4-12