FACS analysis. Panel 1A (FACS analysis of CD3+CD4+ and CD3+CD8+ cells of liver samples): Representative liver samples from C57Bl/6 wild-type (i and ii) and IFN-γ−/− on C57Bl/6 background (iii and iv) mice 12 hr after injection with 12 mg/kg of ConA. The number of mononuclear cells per mouse isolated from ConA injected mice was 1.5 × 106 (C57Bl/6) and 1.8 × 106 (C57Bl/6 IFN-γ−/−), respectively. The number of mononuclear cells per mouse isolated from uninjected C57Bl/6 mice was 5.0 × 104 (v and vi). The values in the upper right corners (i-vi) are percents of CD4+ or CD8+ T cells. Panel 1B: The numbers (×106) of CD4+ (lines) and CD8+ (dots) T cells for wild-type (WT) and IFN-γ−/− mice are shown. There were no significant differences between the numbers of CD4+ or CD8+ T cells isolated from the two strains. As expected, there was near absence of these cells in the uninjected mice. Panel 2A-B (FACS analysis of CD3+NKT+and CD45+CD11b+cells of liver samples). Note the significant number of NKT+ cells (lines) present in the WT mice compared to the IFN-γ−/− mice (p<0.005). There was no significant difference between the numbers of CD11b+ cells (dots) isolated from the two strains. As expected, there was near absence of these cells in the uninjected mice.
Al-Shamsi et al. BMC Gastroenterology 2013 13:6 doi:10.1186/1471-230X-13-6