Additional file 6.
Figure S6.Deletion of Foxp3+Treg is the onset of chronic T cell-mediated intestinal inflammation. (A) IFN-γ and IL-17A production of PBS-treated and DTx-treated Rag1−/− mice after transfer of total CD4+ CELLS FROM Foxp3-GFP-DTR mice of Foxp3-GFP mice. DTx was administered every other day between days 14 and 18. CD4+ cells were extracted from mesenteric lymph nodes and stimulated for 48h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are the tabular representation of the mean values ± SD of the concentration of the distinct secreted cytokines derived from five mice per group each analyzed in duplicate. Statistical significance was analyzed by two-tailed unpaired student’s t-test with a 95% confidence interval. *,p≤0.05. (B) Comparison of the IFN-γ and IL-17A production of CD4+ cells isolated from Foxp3-GFP-DTR mice treated with DTx versus the two different control groups. Data shown are the corresponding p values analyzed by a two-tailed unpaired student’s t-test with a 95% confidence interval.
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Boehm et al. BMC Gastroenterology 2012 12:97 doi:10.1186/1471-230X-12-97