Figure 4.

Deletion of Foxp3+Treg is the onset of chronic T cell-mediated intestinal inflammation. (A) Body weight as a percent of starting weight of PBS-treated and DTx-treated Rag1−/− mice after transfer of total CD4+ cells from Foxp3-GFP-DTR mice or Foxp3-GFP mice. DTx was administered every other day between days 14 and 18. Each group initially contained of 10 mice. Data are represented as mean ± SEM. (B) Survival of PBS-treated and DTx-treated Rag1−/− mice after transfer of total CD4+ cells from Foxp3-GFP-DTR mice or Foxp3-GFP mice. DTx was administered every other day between days 14 and 18. (C) H&E staining and histology score of representative colon sections on day 49 after transfer of total CD4+ cells from Foxp3-GFP-DTR mice or Foxp3-GFP mice. DTx was administered every other day between days 14 and 18. Horizontal lines of the scatter plot are mean values derived from five mice per group. The error bars represent the SEM. Each point represents the histological score of the corresponding mouse. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05. (D) IFN-γ and IL-17A production of PBS-treated and DTx-treated Rag1−/− mice after transfer of total CD4+ cells from Foxp3-GFP-DTR mice of Foxp3-GFP mice. DTx was administered every other day between days 14 and 18. CD4+ cells were extracted from mesenteric lymph nodes and stimulated for 48h. Cytokine concentrations were determined in culture supernatants by ELISA. Data shown are mean values ± SD and derived from five mice per group each analyzed induplicate. Statistical significance was analyzed by two-tailed unpaired student´s t-test with a 95% confidence interval. *, p ≤0.05.

Boehm et al. BMC Gastroenterology 2012 12:97   doi:10.1186/1471-230X-12-97
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