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Open Access Research article

Association of an NFKB1 intron SNP (rs4648068) with gastric cancer patients in the Han Chinese population

Renquan Lu12, Xiang Gao1, Yin Chen1, Jian Ni3, Yongfu Yu4, Sheng Li56 and Lin Guo12*

  • * Corresponding author: Lin Guo jykca@sina.com

  • † Equal contributors

Author Affiliations

1 Department of Clinical Laboratory, Shanghai Cancer Center, Fudan University, Shanghai, 200032, China

2 Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China

3 Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, 200240, China

4 Department of Biostatistics, School of Public Health, Fudan University, Shanghai, 200032, China

5 Department of Biochemistry, Dalian Medical University, Shandong, 116011, China

6 Yulong Biomedical Group, Shanghai, 200233, China

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BMC Gastroenterology 2012, 12:87  doi:10.1186/1471-230X-12-87

Published: 10 July 2012

Abstract

Background

Hyperactivation of nuclear factor-κB (NF-κB) is associated with various types of tumors. This study investigated the susceptibility of the rs4648068 A/G genotype in the intron region of NFKB1 to gastric cancer and the association of this polymorphism with clinicopathologic variables in gastric cancer patients.

Methods

A hospital-based case–control study of 248 gastric cancer patients and 192 control individuals was conducted in Fudan University Shanghai Cancer Center (Shanghai, China). Single nucleotide polymorphism (SNP) rs4648068 genotype in NFKB1 from blood samples of a total of 440 people was analyzed by polymerase chain reaction-based genotyping.

Results

The frequencies of the AA, AG, and GG genotypes of the rs4648068 polymorphism were 31.5%, 47.2%, and 21.3% in the gastric cancer patients and 29.7%, 59.9%, and 10.4% in the control individuals, respectively. We found that the GG genotype was associated with a significantly increased risk of gastric cancer (P = 0.042). Furthermore, among the gastric cancer cases, the rs4648068 GG genotype was associated with high clinical stage (AOR = 2.27, 95% CI: 1.11- 4.66), lymph node involvement (AOR = 2.90, 95% CI = 1.40- 6.03) and serosa invasion (AOR = 2.78, 95% CI = 1.34- 5.75). However, rs4648068 genotypes were not associated with tumor differentiation in gastric cancer patients.

Conclusions

Homozygous rs4648068 GG was associated with an increased risk of gastric cancer, especially for the lymph node status and serosa invasion in Han Chinese population.

Keywords:
NFKB1; Gastric cancer; Single nucleotide polymorphism (SNP); Susceptibility