Cytokine levels in mouse colon homogenates and plasma. Colonic tissue homogenates and plasma levels of IL-1β (A, D), IL-6 (B, E) and IL-17 (C, F) of negative control group (n = 10), TregPMA cell treated group (n = 10) and positive control group (n = 9) were analyzed using a mouse cytokine magnetic bead-based multiplex assay. TregPMA cell treatment has shown to suppress increased levels of colonic IL-1β (A), IL-6 (B) and plasma IL-6 (E) in CD4+CD45RBhigh CD25- cell transferred colitic mice. The data were analyzed using one-way ANOVA, followed by Bonferroni’s Multiple Comparison Test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000.
Majowicz et al. BMC Gastroenterology 2012 12:172 doi:10.1186/1471-230X-12-172