Flow diagram depicting treatment of strain A/J mice with carbon tetrachloride (CCl4). (A) Schema. Mice were treated three times per week for 17 weeks by oral gavage with 0.04 cc of a 40% solution of CCl4 in olive oil or the vehicle control. (B) Experimental observations. Fibrosis was evident 15 weeks after initial CCl4 treatment, and hepatocellular carcinomas (HCCs) were identified macroscopically in 100% of mice after 17 weeks. Mice were classified into three groups depending on the time of sacrifice (Time point I (4-6 weeks), Time point II (15-17 weeks), Time point III (24-30 weeks)). (C) Representative macroscopic pictures of livers from mice sacrificed at Time point III are shown.
Fujii et al. BMC Gastroenterology 2010 10:79 doi:10.1186/1471-230X-10-79