Figure 6.

HIF-1α promotes Bax activation in HK-2 cells. Cells were subjected to normoxia or hypoxia of 16 h after sorting in a flow cytometer. Active and Total Bax protein expression of the whole cell lysates of EGFP positive cells is shown of cells transfected with either pEGFP or pEGFP and pcDNA3-HA-Hif-1α or pcDNA3-DN-Hif-1α. N = Normoxia; H = Hypoxia. Data shown is representative of two separate experiments.