Additional File 2.

Immunoconfocal analysis of a myogenic marker desmin in populations of primary myoblasts at an early stage and a late stage of cultivation. Immunostaining was performed with anti-desmin mouse monoclonal antibody as a primary antibody and goat anti-mouse Cy3-labelled as a secondary antibody. Cells were grown to form a monolayer on glass cover slips and were fixed with ice-cold methanol before immunostaining. Images were obtained using a Leica TCSNT confocal microscope at an instrumental magnification of 800 times. Phase contrast (A) and immunostaining (B) micrographs of primary rat myoblasts obtained after magnetic sorting with anti-α-7 integrin antibody. Phase contrast (C) and immunostaining (D) micrographs of primary rat myoblasts after magnetic sorting with anti-α-7 integrin antibody followed by an additional 4-week passaging to allow transduction with the MLV-CX43-EGFP vector and EGFP-based preparative FACS sorting. Phase contrast (E) and immunostaining (F) micrographs of NIH3T3 mouse fibroblasts (desmin-negative control). Phase contrast (G) and immunostaining (H) micrographs of L6 rat myoblasts (desmin-positive control).

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Tolmachov et al. BMC Cardiovascular Disorders 2006 6:25   doi:10.1186/1471-2261-6-25