Figure 3.

Sirolimus does not stimulate TF release and activity from SMC. Human SMC released much higher TF activity into the culture medium than endothelial cells (HUVECs) (p < 0.0001, n = 6–9). TF activity released from SMC was not further increased by sirolimus (20 ng/ml, 24 hours). The TF activity released from HUVECs was significantly stimulated by TNF-α (20 ng/ml; 5 hours, p < 0.05, n = 9).