Continuous assay for MMP-12. Continuous fluorometric assay of MMP-12 in (A) aortic specimen from AD(n=12) and CAD(n=4), (B) blood serum sample from AD (n=15) and healthy(n=10) , (C) aortic specimens and sera from AD, (D) aortic specime n from AD incubated with MMP Inhibitor v. Assays were performed at RT in a total of 100 μl assay buffer containing various optimized sample volumes, by activating MMPs with 1mM APMA , inhibiting MMPs with 1 μM MMP Inhibitor v, or 100 ng of recombinant MMP-12 served as a control. Fluorescence was read at Ex/Em wavelengths = 340 nm/490 nm for 60 min. RFU: Relative Fluorescence Unit; AD: aortic dissection; CAD: coronary artery disease.
Song et al. BMC Cardiovascular Disorders 2013 13:34 doi:10.1186/1471-2261-13-34