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Open Access Highly Accessed Research article

Combination coating of chitosan and anti-CD34 antibody applied on sirolimus-eluting stents can promote endothelialization while reducing neointimal formation

Feng Yang12, Shi-Chao Feng12, Xiang-Jun Pang3, Wei-Xiao Li12, Yong-Hua Bi12, Qian Zhao1, Shi-Xuan Zhang5, Yang Wang4 and Bo Feng12*

Author Affiliations

1 Interventional Radiology Department, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, Liaoning, PR China

2 Key Laboratory of Diagnostic Imaging and Interventional Radiology, 5 Nanqi Western Road, Shenyang, 110024, Liaoning Province, PR China

3 Fei Cheng Hospital of Traditional Chinese Medicine, 024 Chang Shan Street, Feicheng, 271601, Shandong, PR China

4 Department of Laboratory Animals, General Hospital of Shenyang Military Area Command, No.83, Wenhua Road, Shenhe District, Shenyang, 110840, PR China

5 Dalian University of Technology, No.2 Linggong Road, Ganjingzi District, Dalian City, 116024, Liaoning, PR China

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BMC Cardiovascular Disorders 2012, 12:96  doi:10.1186/1471-2261-12-96

Published: 26 October 2012

Abstract

Background

Circulating endothelial progenitor cells (EPCs) capture technology improves endothelialization rates of sirolimus-eluting stents (SES), but the problem of delayed re-endothelialization, as well as endothelial dysfunction, has still not been overcome. Therefore, we investigated whether the combination coating of hyaluronan-chitosan (HC) and anti-CD34 antibody applied on an SES (HCASES) can promote endothelialization, while reducing neointimal formation and inflammation.

Methods

Sirolimus-eluting stents(SES), anti-CD34 antibody stents (GS) and HC-anti-CD34 antibody combined with sirolimus-eluting stents (HCASES) were deployed in 54 normal porcine arteries and harvested for scanning electron microscopy (SEM) and histological analysis. The ratio of endothelial coverage above the stents was evaluated by SEM analysis at 7, 14 and 28 days. The percentage of in-stent stenosis was histologically analyzed at 14 and 28 days.

Results

SEM analysis at 7 days showed that endothelial strut coverage was increased in the HCASES group (68±7%) compared with that in the SES group (31±4%, p=0.02). At 14 days, stent surface endothelialization, evaluated by SEM, showed a significantly higher extent of endothelial coverage above struts in the GS (95 ± 2%) and the HCASES groups (87±4%) compared with that in the SES group (51±6%, p=0.02). Histological examination showed that the percentage of stenosis in the HCASES group was not significantly different to that of the SES and GS groups (both p> 0.05). At 28 days, there was no difference in the rates of endothelial coverage between the HCASES and GS groups. The HCASES group showed less stenosis than that in the GS group (P < 0.05), but it was not significantly different from the SES group (P=0.068).

Conclusions

SEM and histology demonstrated that HCASESs can promote re-endothelialization while enhancing antiproliferative effects.

Keywords:
Anti-CD34 antibody; Endothelial progenitor cells; Hyaluronan and chitosan coating; Scanning electron microscopy