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Open Access Highly Accessed Research article

Isoflurane reduces hypoxia/reoxygenation-induced apoptosis and mitochondrial permeability transition in rat primary cultured cardiocytes

Wanjun Wu1, Xianju Zhou2, Ping Liu1, Weidong Fei1, Li Li1 and Huifang Yun1*

Author Affiliations

1 Department of Anesthesiology, Changzhou No.2 People’s Hospital, the affiliated hospital of Nanjing Medical University, Changzhou 213003, China

2 Department of Neurology, Changzhou No.2 People’s Hospital, the affiliated hospital of Nanjing Medical University, Changzhou 213003, China

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BMC Anesthesiology 2014, 14:17  doi:10.1186/1471-2253-14-17

Published: 10 March 2014

Abstract

Background

The volatile anesthetic isoflurane protects the heart from hypoxia/reperfusion (H/R) injury. However, it is still incompletely understood whether isoflurane exerts its protective role through preventing mitochondrial permeability transition pore (MPTP) opening.

Methods

Primary cultured cardiocytes were exposed to H/R in the absence or presence of isoflurane. Cell cytotoxicity and apoptosis were detected by MTT assay and TUNEL staining, respectively. MPTP function was monitored by confocal imaging after reoxygenation. ROS production and activation of caspase-3 were determined by fluorescent reader and western blot, respectively.

Results

As compared to the control group, H/R led to significant cell cytotoxicity and apoptosis, while application of isoflurane markedly reversed the effects. Furthermore, isoflurane significantly inhibits the formation of H/R-induced excess ROS production. Finally, isoflurane attenuated the onset of mitochondrial permeability transition pore (MPTP) occurred during hypoxia/reoxygenation, and in turn inhibited activation of caspase-3.

Conclusions

These data indicate that isoflurane has a protective effect on cardiocytes exposed to H/R by reducing excess ROS production, blocking open of MPTP and further reducing apoptosis.

Keywords:
Isoflurane; Hypoxia/reoxygenation; Cardiocyte; Apoptosis; Mitochondrial permeability transition; ROS