Research article

Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection

Sandra Thibivilliers¹ , Trupti Joshi² , Kimberly B Campbell³ , Brian Scheffler⁴ , Dong Xu² , Bret Cooper³ , Henry T Nguyen¹ and Gary Stacey¹

¹National Center for Soybean Biotechnology, Center for Sustainable Energy, Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, MO, 65211, USA

²Computer Science Department and Christopher S Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA

³Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, 20705, USA

⁴MSA Genomics Laboratory, USDA-ARS, Stoneville, MS, 38776, USA

author email corresponding author email

BMC Plant Biology 2009, 9:46doi:10.1186/1471-2229-9-46

Published:	27 April 2009

Abstract

Background

Phaseolus vulgaris (common bean) is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like Uromyces appendiculatus (bean rust), have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized.

Results

A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene Ur-4. The construction of this library allowed the identification of 6,202 new bean ESTs, significantly adding to the available sequences for this plant. Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation) mainly due to the presence of the Avr4 gene in the race 49 leading to a hypersensitive response in the bean plants. A biphasic pattern of gene expression was observed for several genes regulated in response to fungal infection.

Conclusion

The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the P. vulgaris-U. appendiculatus pathosystem could serve as a model to explore legume-rust interaction.