Open Access Highly Accessed Research article

Nanoparticle penetration and transport in living pumpkin plants: in situ subcellular identification

Eduardo Corredor15, Pilar S Testillano1, María-José Coronado1, Pablo González-Melendi16, Rodrigo Fernández-Pacheco2, Clara Marquina3, M Ricardo Ibarra23, Jesús M de la Fuente2, Diego Rubiales4, Alejandro Pérez-de-Luque4 and María-Carmen Risueño1*

Author Affiliations

1 Centro de Investigaciones Biológicas, (CIB) CSIC, Ramiro de Maeztu 9, E-28040, Madrid, Spain

2 Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Edificio Interfacultativo II, Pedro Cerbuna 12, 50009, Zaragoza, Spain

3 Instituto de Ciencia de Materiales de Aragón (ICMA)Departamento de Física de la Materia Condensada, CSIC-Universidad de Zaragoza Pedro Cerbuna 12, 50009, Zaragoza, Spain

4 Instituto de Agricultura Sostenible, CSIC, Alameda del Obispo s/n, Apdo, 4084, E-14080, Córdoba, Spain

5 School of Biosciences, University of Birmingham, B15 2TT Birmingham, UK

6 Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, ETS Ingenieros Agrónomos, Ciudad Universitaria s/n, 28040, Madrid, Spain

For all author emails, please log on.

BMC Plant Biology 2009, 9:45  doi:10.1186/1471-2229-9-45

Published: 23 April 2009

Additional files

Additional file 1:

Correlative images 1. Bright and dark field visualization of the nanoparticles shown in Figure 2A: A) Bright field image, of the same field in (Fig. 2A). The nanoparticle aggregates appear as a dark material. B) Dark field image of the same field in (Fig. 2A). The nanoparticles appear as bright refringent. Bar = 40 μm

Format: JPEG Size: 479KB Download file

Open Data

Additional file 2:

Correlative images 2. Bright and dark field visualization, and electron microscopy of the nanoparticles shown in Figure 3B. A) Dark field image, B) Bright field image, C) Electron micrograph of the nanoparticle aggregates of (A) and (B). Bar in A and B = 50 μm; C = 2 μm.

Format: JPEG Size: 1.4MB Download file

Open Data

Additional file 3:

Correlative images 3. Detail of the cell squared in fig 4A. A) Phase contrast image, showing nanoparticle aggregates that are visible as an intense dark material. The cytoplasm appears dense and displaying numerous structures and organelles in comparison with the surrounding cells. B) Same field that in (A), bright field image. Bar = 20 μm.

Format: JPEG Size: 424KB Download file

Open Data

Additional file 4:

Correlative images 4. Bright and dark field visualization of the nanoparticles shown in Figure 7A. A) bright field image. B) dark field image. Bar = 30 μm.

Format: JPEG Size: 377KB Download file

Open Data