Table 2 |
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|
Bin location of gliadin proteins and effect on rheological parameters if absent in deletion lines. |
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|
mAb |
Rheological parameters |
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|
|
|
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|
Gliadin protein bands in CS |
Glia-α9 |
Glia-α20 |
Bin location |
Total protein in flour (%) |
DDTa (min) |
BWPRb (%) |
T1/2c (sec) |
GMPd volume (μl/mg) |
Particle surface area (D3,2) (μm) |
|
|
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|
1 |
yes |
yes |
1DS5-0.70–1.00 |
0 |
0 |
+ |
0 |
+ |
0 |
|
2 |
yes |
yes |
1DS5-0.70–1.00 |
0 |
0 |
+ |
0 |
+ |
0 |
|
3 |
yes |
no |
1DS5-0.70–1.00 |
0 |
0 |
+ |
0 |
+ |
0 |
|
4 |
yes |
no |
1DS5-0.70–1.00 |
0 |
0 |
+ |
0 |
+ |
0 |
|
5 |
yes |
no |
1BSsat0.50–1.00 |
0 |
0 |
+ |
0 |
- |
- |
|
6 |
yes |
no |
1AS3-0.86–1.00 |
+ |
+ |
+ |
+ |
+ |
0 |
|
7 |
no |
yes |
6DS4-0.79–0.99 |
+ |
+ |
+ |
- |
- |
- |
|
8 |
yes |
yes |
6DS4-0.79–0.99 |
+ |
+ |
+ |
- |
- |
- |
|
9 |
yes |
yes |
6DS4-0.79–0.99 |
+ |
+ |
+ |
- |
- |
- |
|
10 |
yes |
no |
6DS4-0.79–0.99 |
+ |
+ |
+ |
- |
- |
- |
|
11 |
yes |
yes |
6AS-0.35–1.00 |
+ |
0 |
++ |
-- |
+ |
++ |
|
12 |
no |
yes |
6AS-0.35–1.00 |
+ |
0 |
++ |
-- |
+ |
++ |
|
|
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|
Chinese Spring wild type |
0 |
0 |
0 |
0 |
0 |
0 |
|||
|
|
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|
Protein bands are numbered as shown in Figure 6 and their bin location is determined. Bin location is linked to rheological parameters. Results are compared to Chinese Spring, which is set at "0". "+" in cell means value is up to 50% higher. "++" in cell means value is between 50 to100% higher. "-" in cell means values is up to 50% lower. "--" in cell means value is between 50 to100% lower. "Yes" or "no" in cell means the reaction with the mAb. aDough development time bBand Width at Peak Resistance cFlow-relaxation half time dGlutenin Macro Polymer |
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|
van den Broeck et al. BMC Plant Biology 2009 9:41 doi:10.1186/1471-2229-9-41 |
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