Additional file 1:

Additional Figures S1-S5. Figure S1: Sequence alignment of EUL sequences with known lectin sequences. (A, B) Alignment of the amino acid sequences of EEA and the individual ricin-B domains of Ricinus communis agglutinin (AAA33869.1). (C) Alignment of the amino acid sequences of the EUL protein from Curcuma longa (CurloEULS3) and the N-terminal sequence and two tryptic peptides of the tulip lectin TxLMI. The N-terminal sequence of TxLMI is shown in bold. Figure S2: Multiple sequence alignment of the amino acid sequences of the EUL domain of Euonymus europaeus and the S3-type EULs from different plant species. The lectin abbreviations can be found in Additional file 3: Table S2. Identical residues are indicated by asterisks and similar residues by dashes or colons. The percentage sequence identity/similarity of each protein with EEA is also shown. Figure S3: Expression profile of the EUL from Arabidopsis thaliana (At2 g39050, ArathEULS3) based on the data provided by the Arabidopsis eFP browser. Relative expression of ArathEULS3 in the shoots of 18 day-old seedlings subjected to different abiotic stresses (A) and treatments with plant hormones (B). Relative expression of ArathEULS3 in leaves of 4 week-old plants after infection with the pathogens Botrytis cinerea (C), Pseudomonas syringae pv. tomato DC3000 (avirulent strain) and Pseudomonas syringae pv. tomato avrRpm1 (virulent strain) (D). Figure S4: Alignment of EST sequence from Aedes aegypti (Aedae) and a nearly identical sequence from creeping bentgrass (Agrostis stolonifera) (Agrst). Identical nucleotides are indicated by asterisks. Figure S5: Amino acid sequences of proteins containing one or two Euonymus lectin (EUL) domains. The EUL domains are shaded yellow and green. Signal peptides are shaded grey. Only the EUL domains were used for construction of the phylogenetic tree shown in Figure 6. The first EUL-domain and the second EUL-domain of the two-domain lectins are indicated in the tree with d1 and d2, respectively. Accession numbers can be found in the Additional file 3: Table S2.

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Additional file 2:

Additional data from transcriptome analyses.

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Additional file 3:

Table S1 and S2. Table S1: List of metazoan EST sequences encoding proteins with an EUL domain. Table S2: Overview of sequences encoding single (S)- and double (D)-domain EULs used to construct a phylogenetic tree (Figure 6). Sequences for all EUL proteins are shown in Additional file 1: Figure S5. v: vacuolar EUL homologs.

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